Computational Biology CB_M0002

title:Temperature-Dependent Intermediates in HIV-1 Envelope Glycoprotein-Mediated
Fusion Revealed by Inhibitors that Target N-and C-Terminal Helical Regions of
HIV-1 gp41
author:Stephen A. Gallo, G. Marius Clore, John M. Louis, Carole A. Bewley,and Robert Blumenthal
year:2004
place of publish:

abstract:
Peptides derived from the N- (N-HR) and C- (C-HR) terminal heptad repeat regions adjacent
to the fusion peptide and transmembrane domains, respectively, of human immunodeficiency virus (HIV)-1
gp41 inhibit HIV-1 viral envelope glycoproteins (Env)-mediated cell fusion specifically. The mechanism
of HIV-1 Env-mediated cell fusion and its inhibition by agents that target the N- and C-HR regions was
investigated. Priming experiments with Env-expressing cells indicate that the N-HR region but not the
C-HR region is exposed by treatment with sCD4 at 31 °C, whereas both the N- and C-HR regions are
exposed at 37 °C