Genomics GM_Q0006

Title : Automated multi-dimensional purification of tagged proteins
Author : Jill A. Sigrell, P?r Eklund, Markus Galin, Lotta Hedkvist, Pia Liljedahl, Christine Markeland Johansson,Thomas Pless and Karin Torstenson
Year Publish : 2003
Place of Publish : Springer Netherlands
Abstract :

The capacity for high throughput purification (HTP) is essential in fields such as structural genomics where large numbers of protein samples are routinely characterized in, for example, studies of structural determination, functionality and drug development. Proteins required for such analysis must be pure and homogenous and available in relatively large amounts. ?KTAT 3D system is a powerful automated protein purification system, which minimizes preparation, run-time and repetitive manual tasks. It has the capacity to purify up to 6 different His6- or GST-tagged proteins per day and can produce 1–50 mg protein per run at >90% purity. The success of automated protein purification increases with careful experimental planning. Protocol, columns and buffers need to be chosen with the final application area for the purified protein in mind.