Bioinformatics BI_Q0002

Title : Quantitative analysis of histone exchange for transcriptionally active chromatin
Author : Stephanie D Byrum1, Sean D Taverna2† and Alan J Tackett1
Year Publish : 2011
Place of Publish:  Journal of Clinical Bioinformatics
Abstract :

Genome-wide studies use techniques, like chromatin immunoprecipitation, to purify small chromatin sections so that protein-protein and protein-DNA interactions can be analyzed for their roles in modulating gene transcription. Histone post-translational modifications (PTMs) are key regulators of gene transcription and are therefore prime targets for these types of studies. Chromatin purification protocols vary in the amount of chemical cross-linking used to preserve in vivo interactions. A balanced level of chemical cross-linking is required to preserve the native chromatin state during purification, while still allowing for solubility and interaction with affinity reagents